Immunostaining Photomicrographs
Type I collagen immunostaining of a temporal bone from an individual with normal hearing
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Figure 1A: Organ of Corti (OC), stia vascularis (SV), and spiral ligament (SL) in middle turn of the cochlea. |
Figure 1B: Spiral ganglion (GC) in modiolus and nerve fibers (NF) entering spiral lamina in middle turn of the cochlea. |
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Figure 1C: Crista ampullaris |
Figure 1D: Promontory bone. |
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Figure 1F: Human skin. Positive collagen I staining of the dermis (D) and no staining of the epidermis (E) is observed. |
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Confocal micrographs of a temporal bone tissue from a normal hearing individual immunostained with an anti-COXIII primary antibody and CY-5 labeled secondary antibody
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Figure 2A: Organ of Corti (OC), stria vascularis (SV), and spiral ligament (SL) in middle turn of the cochlea. |
Figure 2B: Spiral ganglion (SG) and nerve fibers (NF) in modiolus. |
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Figure 2D: Negative control with the primary COX III antibody omitted. A- Stria vascularis (SV) and spiral ligament (SL) in basal turn of the cochlea near the hook. |
Confocal micrographs of TUNEL stained tissue. Spiral ganglion in a temporal bone from a 76 year old individual counterstained with DRAQ5 nuclear dye
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Figure 3A: Basal turn. |
Figure 3B: Middle turn. |










