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Immunostaining Photomicrographs

Type I collagen immunostaining of a temporal bone from an individual with normal hearing

imfig1a

Figure 1A: Organ of Corti (OC), stia vascularis (SV), and spiral ligament (SL) in middle turn of the cochlea.

imfig1b

Figure 1B: Spiral ganglion (GC) in modiolus and nerve fibers (NF) entering spiral lamina in middle turn of the cochlea.

imfig1c

Figure 1C: Crista ampullaris
of lateral semicircular canal.

imfig1d

Figure 1D: Promontory bone.

imfig1f

Figure 1F: Human skin.  Positive collagen I staining of the dermis (D) and no staining of the epidermis (E) is observed.

 

 

Confocal micrographs of a temporal bone tissue from a normal hearing individual immunostained with an anti-COXIII primary antibody and CY-5 labeled secondary antibody

imfig2a

Figure 2A: Organ of Corti (OC), stria vascularis (SV), and spiral ligament (SL) in middle turn of the cochlea.

imfig2b

Figure 2B: Spiral ganglion (SG) and nerve fibers (NF) in modiolus.

imfig2c
Figure 2C: High magnification view
of the spiral ganglion with
intense COX III staining in the cytoplasm (C) and no staining
in the nucleus (N).

imfig2d

Figure 2D: Negative control with the primary COX III antibody omitted.  A- Stria vascularis (SV) and spiral ligament (SL) in basal turn of the cochlea near the hook.

Confocal micrographs of TUNEL stained tissue.  Spiral ganglion in a temporal bone from a 76 year old individual counterstained with DRAQ5 nuclear dye

imfig3a

Figure 3A: Basal turn.

imfig3b

Figure 3B: Middle turn.