John Alverdy, MD, has run a continuously funded NIH-funded laboratory that studies the molecular interactions of bacteria and the intestinal mucosa in order to understand how life-threatening infections arise after trauma and major surgery and during critical illness. He has developed several anti-infective polymer-based compounds that can attenuate the virulence of several multi-drug resistant pathogens that cause life threatening infections in surgical patients and works with the IME to synthesize, refine, and scale the compounds for pre-clinical testing.
The Alverdy lab seeks to better understand the regulation of virulence expression among potential pathogens through investigating the characteristics of the microbial context, molecular machinery that senses that context, and ultimately the lethal combinations of virulence expression that leads to disease. The majority of our work has focused on the sense and response virulence mechanisms of Pseudomonas aeruginosa, a well characterized and clinically important pathogen. We have shown a remarkable potential for this organisms to respond to host environmental cues related to stress, ischemia, immune activation and nutrient depletion. With this core model of environmental regulation of virulence expression, we are pursuing applications in intestinal transplantation, anastomotic and radiation physiology, necrotizing enterocolitis and ischemia/reperfusion injury. We are also investigating similar sense and response mechanisms in other clinically important organisms, including Staphylococcus aureus and Candida albicans. Finally, we are interested in developing virulence-based therapies to prevent virulence activation through modifications in microenvironment of the stressed host such as phosphate repletion and polymer-mediated mucosal replacement therapies.
The ultimate goal of understanding microbial virulence is to provide clinical tools to improve the care of patients. However the complexity of the host-pathogen interaction and the vast amounts of mechanistic information available constitutes a formidable barrier to translational research. Computational agent based modeling is a well suited to dynamically represent mechanistic detail in a modifiable context to recapitulate cellular behavior at the tissue, organ and patient levels.
Michael Reese Hospital and Medical Center
Residency in Surgery
University of California
Trauma & Burn Fellowship
Loyola University, Stritch School of Medicine
Prevention of Anastomotic Leak Via Local Application of Tranexamic Acid to Target Bacterial-mediated Plasminogen Activation: A Practical Solution to a Complex Problem.
Jacobson RA, Williamson AJ, Wienholts K, Gaines S, Hyoju S, van Goor H, Zaborin A, Shogan BD, Zaborina O, Alverdy JC. Prevention of Anastomotic Leak Via Local Application of Tranexamic Acid to Target Bacterial-mediated Plasminogen Activation: A Practical Solution to a Complex Problem. Ann Surg. 2021 12 01; 274(6):e1038-e1046.
Influence of the Microbiome on Anastomotic Leak.
Williamson AJ, Alverdy JC. Influence of the Microbiome on Anastomotic Leak. Clin Colon Rectal Surg. 2021 Nov; 34(6):439-446.
Case presentation and panel discussion: Critical illness.
Mundi MS, Patel JJ, Mechanick JI, Arabi YM, Alverdy J, Puthucheary Z, McClave SA. Case presentation and panel discussion: Critical illness. JPEN J Parenter Enteral Nutr. 2021 11; 45(S2):79-84.
Re-examining chemically defined liquid diets through the lens of the microbiome.
Toni T, Alverdy J, Gershuni V. Re-examining chemically defined liquid diets through the lens of the microbiome. Nat Rev Gastroenterol Hepatol. 2021 12; 18(12):903-911.
Autologous Posterior Rectus Sheath as a Vascularized Onlay Flap: a Novel Approach to Hiatal Hernia Repair.
Vigneswaran Y, Bryan AF, Ruhle B, Gottlieb LJ, Alverdy J. Autologous Posterior Rectus Sheath as a Vascularized Onlay Flap: a Novel Approach to Hiatal Hernia Repair. J Gastrointest Surg. 2022 01; 26(1):268-274.
Spatioregional assessment of the gut microbiota in experimental necrotizing pancreatitis.
van den Berg FF, Hugenholtz F, Boermeester MA, Zaborina O, Alverdy JC. Spatioregional assessment of the gut microbiota in experimental necrotizing pancreatitis. BJS Open. 2021 09 06; 5(5).
Enterococcus faecalis Is Associated with Anastomotic Leak in Patients Undergoing Colorectal Surgery.
Anderson DI, Keskey R, Ackerman MT, Zaborina O, Hyman N, Alverdy JC, Shogan BD. Enterococcus faecalis Is Associated with Anastomotic Leak in Patients Undergoing Colorectal Surgery. Surg Infect (Larchmt). 2021 Dec; 22(10):1047-1051.
Sepsis and the Microbiome: A Vicious Cycle.
Miller WD, Keskey R, Alverdy JC. Sepsis and the Microbiome: A Vicious Cycle. J Infect Dis. 2021 06 16; 223(12 Suppl 2):S264-S269.
Anastomotic Leak: Toward an Understanding of Its Root Causes.
Alverdy JC, Schardey HM. Anastomotic Leak: Toward an Understanding of Its Root Causes. J Gastrointest Surg. 2021 11; 25(11):2966-2975.
Bowel preparation in colorectal surgery: the day of reckoning is here.
Alverdy JC. Bowel preparation in colorectal surgery: the day of reckoning is here. Br J Surg. 2021 04 30; 108(4):340-341.